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. 2019 May 21;13:108. doi: 10.3389/fnhum.2019.00108

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Copyright © 2019 Cheng, Tan, Zhu, Zhang, Han, Fang, Jin and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lysosome homeostasis disruption accompanied by TFEB fails to transport to the nucleus. After treatment with 15 μM or 30 μM midazolam, the levels of TFEB at different times in the nucleus were examined. (A,C) Western Blot results of nuclear TFEB level in HEK293 cells. (B,D) Statistical results of (A,C), respectively. (E,G) Western Blot results of nuclear TFEB level in HeLa cells. (F,H) Statistical results of (B,D), respectively (Mean ± SEM. N = 3. **p < 0.01, ***p < 0.001, ns: no significant).