Figure 3.

Midazolam prevents TFEB transport to the nucleus in the starved cell. Starvation 4 h as a positive control, is known to facilitate the intervention of TFEB into the nucleus. Before starved for 4 h, cells were pretreated with or without 30 μM midazolam for 24 h. (A,B) Western Blot results of nuclear TFEB levels in HEK293 cells and HeLa cells, respectively. (C,D) Statistical results of nuclear TFEB levels related to H3 levels in HEK293 cells and HeLa cells, respectively. (E) Representative fluorescent pictures of GFP-TFEB/HeLa cells nucleus translocation. The nucleus was stained by DAPI. Scale bar = 20 μM. C Control, Mida: Midazolam, St: Starvation (Mean ± SEM. N = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant).