Figure 5.

Knockdown of α2δ1 inhibited cancer stem cell properties of α2δ1⁺ HGC-27 cells. (A) Western blotting showed the difference of α2δ1, CD44 and ALDH expression between unmediated α2δ1⁺ HGC-27 cells (NC) and shα2δ1 HGC-27 cells. α2δ1 was confirmed downregulated in shα2δ1 HGC-27 cells, while CD44 and ALDH remained at the same level. (B) Comparison of tumor-formation frequency of unmediated α2δ1⁺ HGC-27 cells (NC) and shα2δ1 HGC-27 cells in NOD/SCID mice. The tumorigenic capacity was significantly inhibited after α2δ1 was knocked down. (C) Comparison of sphere-formation frequency of unmediated α2δ1⁺ HGC-27 cells (NC) and shα2δ1 HGC-27 cells in vitro. The sphere-formation capacity was significantly inhibited after α2δ1 was knocked down. (D) Comparison of IC₅₀ between unmediated α2δ1⁺ HGC-27 cells (black) and shα2δ1 HGC-27 cells (red) when treated with cisplatin. *p<0.05.

Abbreviation: NC, negative control; CDDP, cisplatin.