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. 2019 May 23;14:3831–3843. doi: 10.2147/IJN.S206313

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© 2019 Yin et al.

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Cell adhesion, proliferation, and morphological analysis of rBMMSCs on sample disks.Notes: (A and B) TNS and TNS-MAP disks were incubated with rBMMSCs and cell adhesion and proliferation were evaluated after 15 mins, 30 mins, 1 day, 3 days and 7days, respectively, with a CellTiter-Blue^® Cell Viability Assay (Promega, Madison, WI, USA), (C and D) TNS and TNS-MAP disks were incubated with rBMMSCs for 6 hrs, stained with phalloidin (F-actin) and DAPI (nuclei), and visualized by fluorescence microscopy; bar =100 μm. (***P<0.001; **P<0.01).

Abbreviations: rBMMSCs, rat bone marrow mesenchymal stem cells; TNS, titanium with nanonetwork structures; TNS-MAP, titanium with nanonetwork structures coated with mussel adhesive protein.