Figure 4.

Expression status of key proteins involved in cell survival, DNA damage, and apoptosis in treated and control MCF7 cells.

Notes: (A) Expression of survivin and γH2AX was assessed in lysates prepared from cells treated with E₂, doxorubicin (Dox), or both for 24 and/or 48 hours; the same nitrocellulose membrane was successively reacted with and stripped of secondary antibodies corresponding to the two proteins. (B) Calculated mean ± SD fold change in protein-expression levels in treated and untreated cells based on three separate experiments.