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. 2019 May 14;2019:5406468. doi: 10.1155/2019/5406468

Figure 3.

Figure 3

Observation of myocardial morphological structure, cell proliferation, apoptosis, and fibrosis in offspring rats. (a) Representative left ventricle sections stained by HE in the normal (control), hypoxia (Hpx), SPD-treated (Hpx-Spd), and SPD+polyamine synthesis inhibitor- (Hpx-Spd-DMFO-) treated groups. (b) Representative immunohistochemical staining for protein expression and localization of MCM2-positive cardiomyocytes. (c) Brown-stained nuclei indicate TUNEL-positive cells. (d) Evaluation of the percentage of binucleated cardiomyocytes (n = 6). (e) Evaluation of the percentage of MCM2-positive cells (n = 10). (f) The percentage of TUNEL-positive nuclei in different groups (n = 8). (g) BAX and BCL2 protein expression detected by western blotting. (h) Quantification of the BAX and BCL2 protein level ratio (n = 4). (i) Representative Masson's trichrome staining in ventricle sections in each group. (j) Evaluation of interstitial fibrotic areas in ventricle sections in each group (n = 8). Data are shown as the mean ± SEM. ∗ P < 0.05 versus control, # P < 0.05 versus the Hpx group, and △ P < 0.05 versus the Hpx-Spd group.