Figure 2.
ET-1 sensitizes TRPA1 channel currents in HEK293 cells expressing TRPA1 and ETAR. (a) Representative inward current traces recorded at –80 mV in whole-cell configuration by patch clamp. MO (5 µM) and ET-1 (100 nM) were applied as indicated. (b) Current–voltage (I–V) curve recorded from HEK293 cell shown in the lower panel of (a). The letters a, b, c denote corresponding time point recorded in lower panel of (a). (c) Summarized data showing MO-activated TRPA1 currents were sensitized after ET-1 pretreatment. Currents were normalized to values first induced by MO application in the absence of ET-1. Inward and outward TRPA1 currents were recorded at –80 and +80 mV, respectively. n = 6 cells/group. **p < 0.01 versus control group.
ET-1: endothelin-1; MO: mustard oil.