Fig. 5.

Breast cancer cells cocultured with adipocytes show altered exosomal miRNA expression profiles. a Exosomes originating from CA-CM viewed by electron microscopy (scale bar, 200 nm). b Exosomes from CA-CM were analysed by western blot. c NanoSight analysis of exosomes derived from MDA-MB-231 cells and CA-CM. d Labelled tumour cell-secreted exosomes (red) were incubated with the indicated adipocytes. The adipocytes were treated with and 20 μg of exosomes purified from cancer medium (CM). e miRNA microarray analysis of purified exosomes from MDA-MB-231 cells and CA-CM. The heatmap shows the top 20 differentially expressed miRNAs between the two groups. f MDA-MB-231 or MCF-7 cells were cocultivated in the presence or absence of adipocytes. After 3 days, exosomal miRNAs were further verified by qPCR. g Adipocytes were cocultivated in the presence or absence of tumour cells. After 3 days, RNA was extracted from the adipocytes and subjected to qPCR analysis with primers specific to mature miRNA. Data are presented as the mean ± S.D. of at least three independent experiments. * P < 0.05 versus control values