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. 2019 May 27;15:1744806919850383. doi: 10.1177/1744806919850383

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Effects of SPS on Npas4 expression in spinal cord after incision surgery. (a) Protein levels of Npas4 in rats’ lumbar spinal cord were analyzed by western blotting. Npas4 protein levels were normalized by β-actin (n = 3). (b) Rats were sacrificed on days 1, 7, 14, and 28 after stress to measure the mRNA levels of Npas4 in the lumbar spinal cord (n = 4). (c) Double immunostain with NeuN (green) and Npas4 (red) in the dorsal horn of spinal cord (scale bar = 50 μm). Data are presented as mean ± SD. **p < 0.01, *p < 0.05 versus control group; statistical analysis was performed using one-way ANOVA followed by the Bonferroni test. SPS: single prolonged stress; Npas4: neuronal PAS domain protein 4; ANOVA: analysis of variance.