Figure 9.

Effects of CORT, RU486, and their combination on Npas4 and GABAergic markers in vitro. (a) Npas4 was rapidly induced after the addition of corticosterone. Separate groups of neuron in vitro were harvested at 0 h, 2 h, 4 h, 8 h, 12 h, or 24 h after stimuli. (b–f) Neuron was harvested after stimulation with CORT or RU486 for 24 h, and mRNA levels of NPAS4, GAD65, GAD67, GABAA α1, and GABAA γ2 were analyzed by quantitative RT-PCR. Data are expressed as mean ± SD (n = 3), ***p < 0.001, **p < 0.01, and *p < 0.05 versus dimethyl sulfoxide group; ^###p < 0.001, ^##p < 0.01, and ^#p < 0.05 versus CORT group. Statistical analyses were performed using one-way ANOVA followed by the Bonferroni test. ANOVA: analysis of variance; CORT: corticosterone; Npas4: neuronal PAS domain protein 4; SD: standard deviation; DMSO: dimethyl sulfoxide; RT-PCR: real-time polymerase chain reaction.