Fig. 5.

Depletion of TIM-1 in NSCLC cell lines. a The expression of TIM-1 at the mRNA level in the LV-TIM-1-shRNA group was significantly lower than that in the LV-NC group, both in A549 (P < 0.01) and SK-MES-1 cells (P < 0.05). b, c We also confirmed that the expression of TIM-1 at the protein level in the LV-TIM-1-shRNA group was significantly lower than that in the LV-NC group, both in A549 (P < 0.001) and SK-MES-1 cells (P < 0.001). d In A549 cells, the cell viability of the LV-TIM-1-shRNA group at 72 h (P < 0.05) or 96 h (P < 0.001) was significantly lower than that of the LV-NC group. e In SK-MES-1 cells, the cell viability of the LV-TIM-1-shRNA group at 48 h (P < 0.05), 72 h (P < 0.01) or 96 h (P < 0.01) was significantly lower than that of the LV-NC group. f, g The cell cycle examination also showed that after depletion of TIM-1, the proportion of G1-phase cells in the LV-TIM-1-shRNA group was significantly increased compared with the LV-NC group, both in A549 and SK-MES-1 cells