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. 2019 May 27;20:32. doi: 10.1186/s40360-019-0312-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The expressions of Nr3c1 in the Leydig cell lineage and the effects of DEX on Leydig cell gene expressions. Stem (SLC), progenitor (PLC), immature (ILC), adult (ALC) Leydig cells were isolated from 6-, 21-, 35-, and 90-day-old rat testes, respectively. Seminiferous tubules were cultured in the LIM in the presence of DEX alone or with RU38486 or RU38486 alone for 21 days, then the qPCR measured mRNA levels of Leydig cell-specific genes (n = 4–6). D10 = DEX 10 nM, D100 = DEX 100 nM, R = RU38486 1 μM. The data are presented as the mean ± SEM. Identical letters showed no significant difference between two groups at P < 0.05