Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 28;38:224. doi: 10.1186/s13046-019-1229-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — RFNs suppress viability and induce apoptosis of imatinib sensitive and resistant cells. Cells were transfected with gRNA-17 plus donor, RFNs-half plus donor, RFNs plus donor, respectively. a Evaluation of cell proliferative capacity by CCK-8 assay. b, c The capacity of colony formation was assessed by colony-forming assay. d Cell nucleuses were stained by DAPI to detect the morphologic changes caused by apoptosis. The white arrows pointed out the typical apoptotic cells. e Satistical analysis result of apoptotic cells tested by flow cytometry. f The activation of apoptotic pathway was investigated by western blot. The results are presented as the means ± SD. One-way ANOVA analysis was used to compare treated groups with control group, p < 0.01 (**) and p < 0.001 (***)