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. 2019 May 28;19:221. doi: 10.1186/s12870-019-1814-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Spatial and temporal stimulation of endocytosis in rhizodermal cells of accession A17 LRs in response to PEG treatment. Representative confocal images of rhizodermal cells of untreated (CTL) or treated roots with 15% PEG (PEG) after (a) 10–15 min or (b) 60 m in the three root zones (see Fig. 2). For each zone a magnification of inset is shown. Arrowheads indicate endocytic fluorescent dots. c, d Quantification of the endocytosis expressed in number of fluorescent dots per 100 μm of PM after 10–15 min (c) or 60 min (d) in control (grey bars) or PEG-treated cells (white bars) according to cell type zone. Values are the means ± SE of 4–6 independent replicates (6–9 roots). Asterisks (*) represent statistical significance of Mann-Whitney test (p < 0.05)