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. Author manuscript; available in PMC: 2019 May 28.
Published in final edited form as: Prostate. 2018 Jul 15;78(15):1201–1212. doi: 10.1002/pros.23695

FIGURE 5.

FIGURE 5

Identification of amino acids residues important for ELL2 turnover. A, C4-2 cells were transfected with FLAG-tagged ELL2 deletion mutants, ELL2 (1-292), ELL2 (293-531), or ELL2 (532-640), for 24 h and then treated with CHX (50 μg/mL) for 0, 4, 8, 12, and 24 h. B, C4-2 cells were transfected with the ELL2 deletion mutants as described in A. and then treated with CHX at 50 μg/mL for 0, 1, 2, 3, and 4 h. C. C4-2 cells were transfected with ELL2 (532-640) deletion mutants and treated with CHX at 50 μg/mL for 0, 15, 30, 45, 60, and 75 min. D. C4-2 cells were transfected with wild-type ELL2 or ELL2 substitution mutants, K571R, K581R, or K599R for 24 h and then treated with CHX at the 50 μg/mL for 0, 6, 12, and 24 h. The whole cell lysates were analyzed by immunoblotting using anti-FLAG antibody. GAPDH was immunoblotted as a loading control.