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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Nutr Biochem. 2019 Mar 11;67:201–211. doi: 10.1016/j.jnutbio.2019.02.013

Figure 1. Effects of PA on the lipid accumulation and the expression of adipogenic markers in differentiated 3T3-L1 cells.

Figure 1.

3T3-L1 preadipocytes were induced into beige adipocytes through the treatment of PA for 8 days. (A) Confluent 3T3-L1 cells were cultured in the differentiation medium with or without PA for 2 days and then switched to the basic medium including insulin and T3 with or without PA for 6 days. (B) Oil-Red O staining of differentiated 3T3-L1 white adipocytes. Bar, 100 μm. (C) The absorbance of the stained Oil-Red O at 530 nm. (D) Western blotting analysis of adipogenic markers FABP4 and PPARγ in the differentiated 3T3-L1 white adipocytes. β-actin was used as the control. (E) Relative quantitative analysis of protein bands. Data are shown as means ± SD of three independent experiments. **p≤0.01 versus control; ***p≤0.001 versus control.