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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Nutr Biochem. 2019 Mar 11;67:201–211. doi: 10.1016/j.jnutbio.2019.02.013

Figure 5. PPARα antagonism inhibits PA-stimulated browning of white adipocytes.

Figure 5.

3T3-L1 preadipocytes were induced into beige adipocytes through the treatment of PA or GW6471 for 8 days. (A) The PPARα antagonist GW6471 inhibits PA- mediated browning effects. Oil-Red O staining of differentiated 3T3-L1 white adipocytes. Bar, 100 μm. (B) The absorbance of the stained Oil-Red O at 530 nm. (C and E) Western blotting analysis of PGC1α, UCP1, PPARα, PRDM16 (C) and p-AMPK/AMPK (E) in beige adipocytes. β-actin was used as the control. (D and F) Relative quantitative analysis of protein bands. Data are shown as means ± SD of three independent experiments. *p≤0.05 versus control; **p≤0.01 versus control; ## p ≤0.01 versus PA treatment; ### p ≤0.001 versus PA treatment.