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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Nutr Biochem. 2019 Mar 11;67:201–211. doi: 10.1016/j.jnutbio.2019.02.013

Figure 6. PPARα is necessary for the effect of PA on beige adipocyte biogenesis.

Figure 6.

3T3-L1 preadipocytes with knockdown of PPARα were induced into beige adipocytes through the treatment of PA for 8 days. (A) The target sequences used for shRNA-mediated experiments. (B) Quantitative real-time PCR analysis of Pparα (left) and western blot analysis of PPARα (right) in shRNA control and shRNA PPARα 3T3-L1 cells. (C) Oil-Red O staining of differentiated 3T3-L1 cells. Bar, 100 μm. (D) The absorbance of the stained Oil-Red O at 530 nm. (E, G and I) Western blotting analysis of brown adipogenic markers (E), PPARγ (G), AMPK and p-AMPK (I) contents in the indicated cells. β-actin was used as the control. (F, H and J) Relative quantitative analysis of protein bands. *p≤0.05 versus control; **p≤0.01 versus control; ns indicates no significant differences between PA treatment and the control group.