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. 2019 Apr 19;42(5):426–439. doi: 10.14348/molcells.2019.0040

Fig. 5. Northern analysis of effects of DsrA on rpoS-lacZ mRNA accumulation in Δ3 and Δ3Δhfq cells.

Fig. 5

(A) Total cellular RNA was prepared from 0.02% arabinose- and IPTG-treated cells grown at 37°C, and subjected to Northern blot analysis. Cells containing pDsrA were treated with IPTG at increasing concentrations from 0 to 0.1 mM. The rpoS-lacZ mRNA was probed with an anti-lacZ oligonucleotide and the 23S ribosomal RNA was detected as a loading control. Representative blots are shown. (B) Northern signals were presented in a bar graph. Δ3, arcZ− dsrA− rprA− hfq+; Δ3Δhfq, arcZ− dsrA− rprA− hfq−; ΔaΔr, arcZ− dsrA+ rprA− hfq+; V, vector control. Three Northern experiments were conducted and the mean rpoS-lacZ concentrations ± SD were calculated.