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. 2019 May 27;2(3):e201900385. doi: 10.26508/lsa.201900385

Figure 1. CHAD domain–containing proteins are present in all kingdoms of life.

Figure 1.

(A) Overview of CHAD domain–containing proteins characterized in this study. (B) Genetic loci of genes encoding CHAD domain–containing proteins (in black). Genes coding for polyP-metabolizing enzymes are highlighted in red. The DNA sequence upstream of RcCHAD is missing from contig RCOM_0386220 in the NCBI database. (C) RT-PCR using primers binding to the RcCHAD coding sequence (left) results in a specific product amplified from cDNA from R. communis leaves (right; DNA sequence in Fig S2). (D) Confocal microscopy of transgenic Arabidopsis lines expressing Ubi10p:RcCHAD-mCherry reveals RcCHAD to localize in the nucleus and nucleolus of root and hypocotyl cells (left, scale bars correspond to 20 μm). A Western blot using an anti-mCherry antibody reveals a specific band migrating at the predicted size of the RcCHAD-mCherry fusion protein (63 kD). The Ponceau-stained membrane is shown as loading control below (the major 55-kD band corresponds to RuBisCo).