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. Author manuscript; available in PMC: 2019 May 28.
Published in final edited form as: Anal Chem. 2016 Jun 16;88(13):6718–6725. doi: 10.1021/acs.analchem.6b00883

Figure 1.

Figure 1.

a) Shows the CDMS mass histogram measured for empty capsids separated from AAV8 vectors with a CBA-Luc genome. The bin width is 20 kDa. The inset shows an expanded view of the measured peak (black line). The red line in the inset shows the calculated histogram for homogeneous capsids consisting of only VP3 (60 copies). The width of this peak is due to the experimental uncertainty in the m/z and charge measurements (see text). The blue line in the inset shows the calculated histogram for capsids with a stochastic distribution of VP1, 2, and 3 in the expected 1:1:10 ratio. The black points in a) are a scatter plot of each ion’s charge and mass for ions with masses greater than 3.4 MDa. There is a single charge distribution centered around 155 elementary charges (e). b) Shows the masses and abundances that result for a stochastic distribution of VP1, 2, and 3 in the expected 1:1:10 ratio. The separation between the peaks in this spectrum is too small for the peaks to be resolved in the measured histogram in a).