Table 1.
Compilation of the Expected and Measured Masses and Peak Full Width at Half Maximum (fwhm) (See Text)a
| expected mass (MDa) | measured mass (MDa) | difference (kDa) | expected fwhm (kDa) | measured fwhm (kDa) | |
|---|---|---|---|---|---|
| 60 copies AAV8 VP3 | 3.586b | – | – | 97 | – |
| AAV8 Capsidc | 3.729b | 3.819 ± 16 | +90 | 152 | 195 ± 8 |
| CBA-Luc genome | 1.269d | 1.261 ± 21 | −8 | – | – |
| AAV8/CBA-Lucc | 4.998 | 5.080 ± 13 | +82 | 174 | 208 ± 7 |
| AAV8 Capsidc | 3.729b | 3.704 ± 13 | −25 | 152 | 180 ± 5 155 ± 11e |
| scGFP Genome | 1.389d | 1.392 ± 13 | +3 | – | – |
| AAV8/scGFPc | 5.118 | 5.095 ± 25 | −23 | 177 | 172 ± 15 |
a
Empty AAV8 capsids were measured after separation from CBA-Luc-containing capsids and from a sample which packaged scGFP. The measurements for these two samples of empty capsids are grouped together with the appropriate genome in the table.
b
See text for masses of VP1, VP2, and VP3.
c
For a stochastic mixture of VP1, VP2, and VP3 in an average ratio of 1:1:10.
d
Sequence mass calculated using http://www.bioinformatics.org/sms2/dna_mw.html.
e
For the higher charge cluster of empty capsids in Figure 5.