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Assessment of cardiac inflammation. The presence of macrophages was determined in TBI and age-matched nonirradiated mouse heart left ventricle myocardium. Macrophage numbers/unit area were determined by counting F4/80-positive cells in 16 fields/ section/mouse (200×) of heart tissue sections and normalizing to unit area. n = 4 for 4 and 13 months, nonirradiated and TBI; n = 4 for 18 months, nonirradiated; n = 7 for 18 months, TBI).
