| Reagent | Quantity | Final concentration (10×) |
| NaCl | 58.44 g | 1 m |
| KCl | 1.34 g | 18 mm |
| CaCl2•2H2O | 2.94 g | 20 mm |
| MgCl2•6H2O | 2.03 g | 10 mm |
| HEPES (molecular grade acid, not the sodium salt) | 35.75 g | 150 mm |
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