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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer. 2019 Jan 8;125(8):1267–1280. doi: 10.1002/cncr.31935

Figure 1. Levels of VEGF, IL-8 and IGF1 are elevated and signaling through PI3K/AKT and MAPK/ERK is activated in ovarian cancer xenografts.

Figure 1.

(A) Growth factor/cytokine antibody arrays were performed using cultured cell lysates and xenograft lysates from SKOv3-DIRAS3 and OVCAR8-DIRAS3 ovarian cancer cell lines. Levels of IGF-1 (red circles), IL-8 (blue circles) and VEGF (green circles) were significantly elevated in xenografts when compared with cultured cells (NEG: negative control, POS: positive control). (B) ELISAs were performed to confirm observations in (* p<0.05, ** p<0.01). (A). (C) Phospho-AKT and phospho-ERK levels were increased in xenografts compared with cultured cells. Sections from formalin-fixed, paraffin embedded OVCAR8-DIRAS3 cell pellets and xenografts were examined by immunohistochemistry using antibodies against EpCAM, p-AKT, total AKT, p-ERK and total ERK as indicated.