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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer. 2019 Jan 8;125(8):1267–1280. doi: 10.1002/cncr.31935

Figure 3. Serum starvation decreases the activity of AKT and ERK and enhances DIRAS3-induced autophagy in ovarian cancer cells.

Figure 3.

(A) Serum starvation decreases phosphorylation of AKT and ERK and increases LC3B II / LC3B I conversion in SKOv3-DIRAS3 and OVCAR8-DIRAS3 cells. Cells were treated with or without DOX for 48 hrs and treated with or without serum free media for the final 24 hrs. Cell lysates were collected for Western blot analysis. Numbers below the bands indicate the band intensity compared to control. (B) Serum starvation (S) enhances messenger RNA (mRNA) expression of LCB3 in SKOv3-DIRAS3 and OVCAR8-DIRAS3 cells. Cells were treated with or without DOX for 48 hrs and treated with or without serum free media for the final 24 hrs. RT-PCR was performed to measure mRNA expression of LC3B. The columns indicate the mean and the bars indicate the S.E. (** p<0.01).