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. 2019 May 28;14(5):e0217394. doi: 10.1371/journal.pone.0217394

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© 2019 Sugihara et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A, Neurite growth in SH-SY5Y cells treated with exosomes derived from non-treated Caco-2 cells (Exo-ctrl), from Caco-2 cells treated with 10 mM carnosine (Exo-Car10), and neurite growth in non-treated SH-SY5Y cells (NT) and cells treated with retinoic acid (RA) were shown. B, Neurite growth in SH-SY5Y cells was quantitatively determined using the IN Cell Analyzer 1000. C-E, Expression of neuronal cell marker genes of nestin (C), vimentin (D) and neurofilament medium (NEFM; E) was quantitatively determined by qRT-PCR. Neurite growth in primary mouse cortex neurons treated with exosomes (Exo-ctrl, Exo-Car1 and Exo-Car10) was quantitatively determined using the IN Cell Analyzer 1000 as described above (F).