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. 2019 May 28;14(5):e0217504. doi: 10.1371/journal.pone.0217504

Fig 1. Assays for bactericidal activity.

Fig 1

(a) Pseudomonas aeruginosa (NBRC 13275), (b) Pseudomonas aeruginosa (PAO1), (c) Acinetobacter baumannii (JCM 6841), (d) MRSA (IID 1677). Each type of bacteria was incubated with LYZOX solution (2,000 μg/mL), the mixed solution (lysozyme solution [1,000 μg/mL] and chitosan oligosaccharide [COS] solution [1,000 μg/mL]) or the lysozyme-galactomannan conjugate (LGC) solution (2,000 μg/mL) in saline at 37°C in a water bath for 0 min, 30 min, 60 min and 120 min. The dilutions were plated, and the colonies were counted following growth overnight. The values are the mean ± SEM from four independent experiments. *p<0.05 or **p<0.01 compared with saline; †p<0.05 or ††p<0.01 compared with LGC; ‡p<0.05 or ‡‡p<0.01 compared with the mixture (unpaired t-test). Symbols: circles, saline; squares, lysozyme-chitosan oligosaccharide conjugate (LYZOX); up-pointing triangles, LGC; down-pointing triangles, mixture.