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. 2019 May 28;14(5):e0217504. doi: 10.1371/journal.pone.0217504

Fig 5. ONPG assays.

Fig 5

The measurement of absorbance at 420 nm (A420 nm) can be used to estimate the activity of cytoplasmic β-galactosidase released from bacteria into LYZOX solution using ONPG as the substrate to evaluate inner membrane permeabilization in gram-negative bacteria and plasma membrane permeabilization in gram-positive bacteria. (a) Pseudomonas aeruginosa (NBRC 13275), (b) Acinetobacter baumannii (JCM 6841), (c) MRSA (IID 1677). The values are the mean ± SEM of triplicate measurements. *p<0.05, **p<0.01 (unpaired t-test).