Table 1. List of oligonucleotide primers used to detect virulence genes of B. pseudomallei.
Virulence Determinants | Oligonucleotide sequence 5’-3’ | Amplicon Size | Reference |
---|---|---|---|
BimABm | BimBm F—AGCGCTTCGCGCATCTAC BIMBm R-CGCGTTAAACGCCGTACTTTC |
104bp | 4 |
BimABp | BimBp F- GGAAGCTTTGGCGTGCATAT BimBp R- CCCATGCCTTCCTCGACTAAT |
60bp | 4 |
fhaB3 |
fhaB3 F-GACGCGGCACGTCTGATC fhaB3 R-CGCGGATAAAACTCGGATTG |
58bp | 4 |
LPS A | wbiE_F-TCAAACCTATCCGCGTGTCGAAGT wbiE_R-TCGTCGTCAAGAAATCCCAGCCAT |
195bp | 3 |
LPS B | BUC3396F-AATCTTTTTCTGATTCCGTCC BUC3396RACCAGAAGACAAGGAGAAAGGCCA |
93bp | 3 |
LPS B2 | BURP840_LPSb16-F- AACCGGGTAGTTCGCGATTAC BURP840_LPSb16-R-ATACGCCGGTGTAGAACAGTA |
364bp | 3 |