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. Author manuscript; available in PMC: 2019 May 28.
Published in final edited form as: Cell Rep. 2019 May 21;27(8):2468–2479.e3. doi: 10.1016/j.celrep.2018.12.063

Figure 2. Loss-of-Function Mutations in ami3, pmt, or mprB Suppress htrA Essentiality.

Figure 2.

(A-C) Double-mutant phenotypes of htrA suppressor strains: ami3 (A), pmt (B), and mprB (C). Wild-type Mycobacterium smegmatis (Msm), single suppressor gene deletions, suppressor deletions with htrA deletions, and htrA complemented strains (see Figures S1 and S6) were grown under conditions identical to those in Figure 1, including growth in rich media at 37°C, high temperature (42°C), carbon-nitrogen starvation (PBS-Tween 20), and oxidative stress (0.02% tert-butyl hydroperoxide). Error bars represent SD of the mean.

(D) htrA suppressor mutants exhibit reduced cell length. Single suppressor deletion strains (blue), htrA double-deletion strains (red), and htrA complemented strains (green) were grown to log phase and analyzed for total cell length (see Figure S2). At least 70 cells were quantified in each condition. Dotted black lines indicate median values. ****p < 0.0001.