| Antibodies |
| Rabbit polyclonal anti-Strep-tag II (NWSHPQFEK) |
GenScript |
Cat#89494–724 |
| Rabbit polyclonal anti-FLAG |
Sigma Aldrich |
Cat#F7425 |
| Rabbit polyclonal anti-HtrA |
Helene Botella and Sabine Ehrt |
N/A |
| Mouse polyclonal anti-RpoB |
ThermoFisher Scientific |
Cat#MA1–25425 |
| Bacterial Strains |
|
Escherichia coli TOP10 |
ThermoFisher Scientific (formerly Invitrogen) |
Cat#404010 |
|
Msm mc2 155 |
Laboratory of Barry Bloom |
N/A |
| Full list of strains in Table S2
|
This paper |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| MagStrep type3 XT Beads, 5% suspension |
IBA Life Sciences |
Cat#2–4090-002 |
| Anti-FLAG M2 Magnetic Beads |
Sigma Aldrich |
Cat#M8823 |
| FLAG peptide |
Sigma Aldrich |
Cat#F3290 |
| Concanavalin A from Canavalia ensiformis (Jack bean) peroxidase conjugate |
Sigma Aldrich |
Cat#L6397 |
| Pierce Avidin Agarose |
ThermoFisher Scientific |
Cat#20219 |
| 10X Buffer BXT |
IBA Life Sciences |
Cat#2–1042-025 |
| Deposited Data |
| Full, uncropped Western blots |
This paper |
https://doi.org/10.17632/r7mm9mtt3v.1#folder-26c6e562-7d8d-44c8-a65a-21fa0ae692eb |
| Oligonucleotides |
| Full list of plasmids and strains in Table S2
|
This paper |
N/A |
| Software and Algorithms |
| ImageJ |
National Institutes of Health |
https://imagej.nih.gov/ij/download.html |
