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. 2019 Apr 18;4(8):e126749. doi: 10.1172/jci.insight.126749

Figure 2. Cisplatin reduces mitophagy which PGC1α counteracts.

Figure 2

(A) Color shift in mtKeima indicates mitophagy from green (neutral) to red (acidic). (B–M) PGC1α-KO cells or controls treated with vehicle or cisplatin (10 M, 24 hours). Scale bars: 5 μm. (N) Quantification of mitophagy index for B–M relative to control condition. n = 32, 16, 20, and 12 fields left to right from 3–4 biological replicates per condition. (O) Mitophagy index for PGC1α Tg cells or vector controls treated with vehicle or cisplatin (10 μM, 24 hours). n = 31, 27, 54, and 31 fields left to right from 3–6 biological replicates per condition. (P) PGC1α-KO cells treated with cisplatin (10 μM, 24 hours) ± concurrent nicotinamide mononucleotide (NMN, 1 mM). n = 16, 20, 12, and 20 fields left to right from 3–4 biological replicates per condition. Results analyzed by 2-way ANOVA on biological replicates with P values as indicated.