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. 2019 Apr 18;4(8):e126747. doi: 10.1172/jci.insight.126747

Figure 3. Blood-retina barrier is first disrupted at retinal pigment epithelium and later at retinal vasculature.

Figure 3

(A) Mice were injected with fluorescent probes, and living eyecup preparations were imaged under confocal microscope and then processed for albumin labeling. (B) Representative images of WT and rd10 retinas after infusion of probes. At P20–P21 light fluorescein labeling was detected at the retinal pigment epithelium (RPE) layer in rd10 retina. In WT retina, both lowest weight fluorescein and highest weight accumulated albumin were contained to blood vessels. (C) At P35 in rd10 retina, outer blood-retina barrier (BRB) had increased permeation for both low-weight-molecular probes and large (70-kDa) blood plasma albumin. (D) At P200 in rd10 retina the leak was evident with all probes from both RPE and deep vascular capillaries. Scale bar: 50 μm. (E–G) Quantification of the vascular leak at P20–P21 (E), P35–P40 (F), and P200 (G). Error bars ± SEM. Each measurement was done in 4–5 different mice.