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. 2019 May 2;4(9):e124747. doi: 10.1172/jci.insight.124747

Figure 2. Detailed transmission electron microscopy analysis of glomeruli in SIRPα–deficient mice.

Figure 2

(A) Transmission electron microscopy (TEM) of foot process fusion and effacement of podocytes and the glomerular basement membrane (GBM) (podocytes of n = 4 WT mice and n = 3 Sirpa–/– mice were analyzed). Open arrowheads, foot process fusion; filled arrowheads, foot process effacement; filled arrows, vacuoles. Histograms represent quantification of foot process fusion, effacement of podocytes, and GBM thickness. (B) TEM of the glomerulus of 6-, 12-, and 20-month-old WT and Sirpa–/– mice. Open black arrowheads, microvillus; open red arrowheads, regular rough ER; filled red arrowheads, distension of rough ER; open red stars, regular mitochondria; filled red stars, damaged mitochondria. Histograms represent quantification of microvillus formation, distension of rough ER, and damaged mitochondria in WT versus Sirpa–/– mice. (podocytes of n = 4 WT mice and n = 3 Sirpa–/– mice were analyzed). (C) Lipofuscin accumulation in 6-, 12-, and 20-month-old Sirpa–/– mice (podocytes of n = 4 WT mice and n = 3 Sirpa–/– mice were analyzed). Arrows indicate lipofuscin aggregates. The histogram represents statistical analysis of lipofuscin aggregates. Scale bars: 1 μm. Data represent the mean ± SEM, and P value was analyzed by 2-tailed Student’s t test. *P < 0.05, **P < 0.01.