Skip to main content
. 2019 May 2;4(9):e124747. doi: 10.1172/jci.insight.124747

Figure 8. Promoting autophagy ameliorates PAN-induced proteinuria and renal pathological damage in Sirpa–/– mice.

Figure 8

(A) Proteinuria (mg/l) content within 24 hours of PAN-induced WT and Sirpa–/– mice administrated with or without Rapa (6 mice/group). (B and C) Rapa treatment results in less pathological damage of glomerulus (B) and foot process fusion of podocytes (C) in Sirpa–/– mice. Foot process fusion and effacement are indicated by open and filled arrowheads, respectively. Histogram represents quantification of GBM thickness (glomeruli from 4 mice of each group were analyzed). (D) Rapa induced GFP-LC3–positive autophagosomes in Sirpa–/– mice. The histogram represents levels of autophagosomes in each group. Each group had 5 mice, and at least 8 glomeruli from each mouse were analyzed. (E) Rapamycin inhibited the accumulation of p62 in Sirpa–/– mice. (F) Western blot analysis of glomerulus p62. The histogram represents statistical results from 3 independently performed experiments. Data in A, C, D, and F represent mean ± SEM, and P value was analyzed by ANOVA with Tukey-Kramer test. Scale bars in B, D, and E: 10 μm. Scale bar in C: 1 μm. *P < 0.05, **P < 0.01.