Table 1. Cryo-EM data collection, refinement and validation statistics.
| TRPV3K169A
(EMD-20192) (PDB 6OT2) |
TRPV3K169A 2-APB
(EMD-20194) (PDB 6OT5) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 130,000x | 75,000x |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 40 | 42 |
| Defocus range (μm) | 1–2.5 | 1.25–3 |
| Pixel size (Å) | 1.06 | 1.08 |
| Symmetry imposed | C4 | C4 |
| Initial particle images (no.) | 452,388 | 1,174,521 |
| Final particle images (no.) | 95,184 | 79,006 |
| Map resolution (Å) | 4.1 | 3.6 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Initial model used (PDB code) | 6MHO | 6MHO |
| Model resolution (Å) | 4.1 | 3.6 |
| FSC threshold | 0.143 | 0.143 |
| Map sharpening B factor (Å2) | −120 | −100 |
| Model composition | ||
| Non-hydrogen atoms | 17,332 | 17,800 |
| Protein residues | 2500 | 2492 |
| Ligands | 0 | 4 (2-APB) |
| B factors (Å2) | ||
| Protein | 87.43 | 40.51 |
| Ligand | n/a | 35.66 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.008 | 0.008 |
| Bond angles (°) | 0.868 | 0.833 |
| MolProbity score | 1.64 | 1.24 |
| Clashscore | 5 | 5 |
| Poor rotamers (%) | 0 | 0 |
| Ramachandran plot | ||
| Favored (%) | 92.70 | 97.01 |
| Allowed (%) | 7.30 | 2.99 |
| Disallowed (%) | 0 | 0 |