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. 2019 Apr 18;4(8):e127882. doi: 10.1172/jci.insight.127882

Figure 3. Epitopes of OVA induce tolerance when expressed endogenously.

Figure 3

(A) Peptide 36–43 (SALAMVYL) is present in 2 proteins expressed by mice. Peptide 36–43 was aligned against all nonredundant mouse (taxid:10090) protein sequences. Thirty-nine–amino acid regions of OVA and 2 mouse proteins containing peptide 36–43 are shown. Numbers flanking the sequences indicate the position (from the N-terminus) of the first and the last depicted amino acid. (B) Act-mOVA mice are tolerized toward all epitopes of OVA. C57BL/6J mice (red) or Act-mOVA (blue) were immunized with OVA and CD8+ T cell responses against the indicated epitopes were tested (n = 3; experiment performed 2 times; unpaired t test). (C) Act-mOVA mice are not tolerized toward peptide 208–216. Wild-type C57BL/6J (left panel) or Act-mOVA (right panel) mice were immunized with peptides 27–35, 55–62, 208–216, and 257–264. CD8 responses were tested 7 days after immunizations (n = 3 for both panels; experiment performed 2 times; unpaired t test). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.