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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Anal Chem. 2018 Aug 17;90(17):10450–10456. doi: 10.1021/acs.analchem.8b02356

Figure 5.

Figure 5

DNA melting analysis. (a) Schematic of an annealed PLA device with an embedded 400 by 400 μm channel exposed to a linear thermal gradient. (b) Autofluorescence as a function of material and annealing. CC-PLA, once annealed and shaded with a marker (Annealed PLA-Dark), allowed for rapid visualization of fluorescence emitted by the sample. (c) Fluorescence emission under an applied spatial thermal gradient. Fluorescence was enhanced for clarity. Thresholding clearly reveals temperature dependence. (d) Normalized fluorescence and Tm of the DNA was comparable between the 3D printed chip developed in this work and a commercial instrument (HR-1). Scale bar, 1 mm.