FIGURE 7.

Combination therapy does not further reduce lymphocyte count over ponesimod monotherapy in an acute monophasic experimental autoimmune encephalomyelitis (EAE) model in Lewis rats. Treatment with vehicle, dimethyl fumarate (DMF) (120mg/kg), ponesimod (100mg/kg), or DMF and ponesimod combination was given orally by gavage, once daily, starting on Day 0 of immunization of rats with myelin basic protein (MBP)/Complete Freund’s Adjuvant (CFA). Blood samples were collected on Day 20, 24 hours after the last dosing of Day 19, and at sacrifice on Day 21, one hour after the last dosing. Lymphocyte count was measured using ADVIA 120 blood analyzer (Siemens, Munich, Germany). A) Lymphocyte count, one hour after the last treatment administration: ***p<0.001, ****p<0.0001 vs. vehicle using a Kruskal-Wallis test followed by Dunn’s multiple comparisons test; B) Lymphocyte count, 24 hours after the last treatment administration: ***p<0.001 vs. vehicle using a Kruskal-Wallis test followed by Dunn’s multiple comparisons test; results expressed as mean+standard error of the mean (SEM)