Figure 12.
Microfluidic synthesis of silica-based biomaterials for protein immobilization. (A) Continuous flow microfluidic reactor system used for the immobilization of GOD in silica nanoparticles (a) and the comparison of the stability of immobilized GOD and GOD in solution (b, from i to v are microreactor system using R5 peptide, microreactor system using PEI, batch reactor system, two-step batch reactor system with silica particle synthesis firstly and then GOD immobilization using PEI, and free GOD in solution, respectively). Reproduced with permission from ref.41. (B) Microfluidic fabrication of hierarchical PCL-silica hybrid microbeads for multiprotein coimmobilization (a) and the comparison of the fluorescence intensity of cy3-BSA (red) and FITC-IgG (green) immobilized microbeads. NP: no pore; SP: shallow pore; DP: deep pore. Reproduced with permission from ref.121.