Table 1. Sequence of the primers for gene expression analysis by real-time RT PCR.
| Gene | NCBI reference sequence | Primer sequences (5′–3′) | Product size (base pair, bp) |
|---|---|---|---|
| β-actin | NC_010454 | F: CCCTGGAGAAGAGCTACGAG | 172 |
| R: TCCTTCCTGATGTCCACGTC | |||
| POU5F1 | NM_001113060 | F: AGTGAGAGGCAACCTGGAGA | 166 |
| R: TCGTTGCGAATAGTCACTGC | |||
| SOX2 | NM_001123197 | F; GCCCTGCAGTACAACTCCAT | 216 |
| R: GCTGATCATGTCCCGTAGGT | |||
| NANOG | XM_013993362 | F: AAGTACCTCAGCCTCCAGCA | 202 |
| R: GGCATCCTTGGTGATAGGAA | |||
| CDX2 | NM_001278769 | F: AGCCAAGTGAAAACCAGGAC | 178 |
| R: TGCGGTTCTGAAACCAGATT |
RT, reverse transcription; PCR, polymerase chain reaction; NCBI, National Center for Biotechnology Information, F, forward; R, Reverse.