Table 2. Development of porcine SCNT embryos by caffeine supplementation in manipulation media with four different concentrations.
| Caffeine concentration | No. of reconstructed oocytes | No. of embryos (%; mean ± SEM) developed to | ||||
|---|---|---|---|---|---|---|
| 2-cell | 4-cell | 8-cell | Morula | Blastocyst | ||
| 0 (control) | 95 | 57 (60.1 ± 1.3)* | 48 (50.9 ± 2.0) | 26 (27.6 ± 2.6) | 20 (21.3 ± 3.0) | 15 (16.1 ± 2.8) |
| 1.25 mM | 95 | 68 (71.8 ± 6.7)* | 58 (61.2 ± 7.5) | 41 (42.7 ± 4.1) | 27 (27.9 ± 4.5) | 21 (22.8 ± 4.3) |
| 2.5 mM | 91 | 73 (80.6 ± 6.4)† | 65 (71.4 ± 5.8) | 34 (37.8 ± 8.0) | 19 (20.5 ± 2.1) | 15 (16.2 ± 2.9) |
| 5.0 mM | 96 | 73 (76.4 ± 6.7)* | 63 (65.6 ± 6.0) | 33 (34.8 ± 7.0) | 24 (25.2 ± 7.0) | 18 (19.1 ± 4.0) |
SCNT, somatic cell nuclear transfer; SEM, standard error of the mean; conc., concentration.
*,†Values in the same column with different superscript letters are significantly different (p < 0.05).