. 2019 May 20;20(3):e31. doi: 10.4142/jvs.2019.20.e31

Table 3. Development of porcine SCNT embryos by the delayed activation of three different times with 1.25 mM caffeine supplementation.

Duration of caffeine treatment (h)	No. of reconstructed oocytes	No. of embryos (%; mean ± SEM) developed to
Duration of caffeine treatment (h)	No. of reconstructed oocytes	2-cell	4-cell	8-cell	Morula	Blastocyst
Control (1 h)	95	61 (64.0 ± 3.8)	50 (52.2 ± 6.0)	24 (25.3 ± 2.2)	18 (18.5 ± 3.2)	9 (9.4 ± 1.4)^*
1 h	98	63 (64.2 ± 5.9)	57 (58.1 ± 5.9)	30 (31.0 ± 3.2)	21 (21.6 ± 2.4)	13 (14.0 ± 3.5)^*
2 h	93	63 (67.9 ± 5.9)	51 (54.3 ± 6.7)	30 (31.8 ± 7.4)	21 (22.3 ± 6.5)	10 (11.1 ± 2.0)^*
4 h	88	58 (65.8 ± 3.6)	52 (59.1 ± 4.1)	31 (36.3 ± 4.9)	25 (27.9 ± 5.3)	20 (22.4 ± 6.1)^†

SCNT, somatic cell nuclear transfer; SEM, standard error of the mean.

^*,†Values in the same column with different superscript letters are significantly different (p < 0.05).