Table 5. Preimplantation development of porcine SCNT embryos with caffeine supplement both of oocyte manipulation and 4 h post-fusion based on the results of previous experiments.

Caffeine concentration (mM)	No. of reconstructed oocytes	No. of embryos (%; mean ± SEM) developed to
		2-cell	4-cell	8-cell	Morula	Blastocyst
0	131	117 (89.6 ± 2.9)	101 (77.1 ± 3.6)	39 (29.5 ± 1.9)	24 (18.3 ± 1.3)	20 (15.2 ± 1.2)
1.25	119	102 (85.7 ± 3.0)	85 (71.7 ± 4.5)	35 (29.6 ± 2.6)	23 (19.3 ± 1.7)	19 (16.0 ± 1.6)

SCNT, somatic cell nuclear transfer; SEM, standard error of the mean.