Fig. 3. Protection efficacies of Salmonella-based LI vaccines. All the vaccinated C57BL/6 mice groups were orally challenged with 106.9 50% tissue culture infectious dose LI strain at 2 weeks post-vaccination. The protection against LI was assessed based on the presence of gDNA in stool samples. (A) Protection shown by an individual vaccine strain. (B) Protection shown by a cocktail of all 4 strains. Protection is presented as the number of animals showing gDNA in stool samples to the total number of animals in that group. Five animals were sacrificed at each time-point indicated. The significance of the data was examined using the Kruskal-Wallis test with Dunn's multiple comparison test.
gDNA, genomic DNA; LI, Lawsonia intracellularis; LfliC, Lawsonia flagellin; Lhly, Lawsonia hemolysin.