Fig. 4. Salmonella ompA-specific serum IgG and vaginal sIgA responses in BALB/c vaccinated mice. BALB/c mice were vaccinated with either the co-mixture of 4 vaccine candidates or sterile PBS, and serum and vaginal wash samples were collected at different time points for IgG and IgA analysis, respectively, by an indirect enzyme-linked immunosorbent assay. (A) ompA-specific IgG measured in serum. (B) ompA-specific IgA measured in vaginal wash samples.

Each data point represents mean ± standard deviation of 5 mice per group. The significance of the data was examined using the Kruskal-Wallis test with Dunn's multiple comparison test.

PBS, phosphate buffered saline; Ig, immunoglobulin; sIgA, secretory immunoglobulin A; LfliC, Lawsonia flagellin; Lhly, Lawsonia hemolysin.

^*p < 0.05.