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. 2019 May 8;20(3):e26. doi: 10.4142/jvs.2019.20.e26

Fig. 1. Validation of eight primer pairs by simplex and multiplex PCR assays. To confirm that primer pairs correctly amplified their respective regions they were evaluated in simplex and multiplex PCR assays using (A) E. faecium, (B) E. faecalis, and (C) E. hirae genomic DNA isolated from broiler chickens suffering lameness. Lane M, 100 bp DNA markers; lane 1, multiplex PCR with E. faecium genomic DNA; lane 10, multiplex PCR with E. faecalis genomic DNA; lane 19, multiplex PCR with E. hirae genomic DNA; lanes 2, 11, and 20, simplex PCR with only fsr primers; lanes 3, 12, and 21, simplex PCR with only efm primers; lanes 4, 13, and 22, simplex PCR with only esp primers; lanes 5, 14, and 23, simplex PCR with only cylA primers; lanes 6, 15, and 24, simplex PCR with only cad1 primers; lanes 7, 16, and 25, simplex PCR with only ace primers; lanes 8, 17, and 26, simplex PCR with only gelE primers; lanes 9, 18, and 27, simplex PCR with only asa1 primers.

Fig. 1

PCR, polymerase chain reaction.