Fig. 2. Evaluation of multiplex PCR detection limits using genomic DNA from (A) E. faecalis (harboring fsr, esp, cylA, cad1, ace, gelE, and asa1), (B) E. faecium (harboring efm and cad1), and (C) E. hirae (harboring cad1). Lane M, 100 bp DNA markers; lane 1, 200.0 ng/µL DNA; lane 2, 40.0 ng/µL DNA; lane 3, 8.0 ng/µL DNA; lane 4, 1.6 ng/µL DNA; lane 5, 320.0 pg/µL DNA; lane 6, 64.0 pg/µL DNA; lane 7, 12.8 pg/µL DNA; lane 8, negative control.
PCR, polymerase chain reaction.