Table 1. Sequences of oligonucleotide primers used to detect putative virulence genes in Enterococcus isolates.

Virulence gene	Gene description	Primer	Sequence (5′ to 3′)	Amplicon size (bp)
fsr	Regulator of gelE and sprE expression	Forward primer	CAA GGC ACT ATT TCT TAC TTA GG	1,016
		Reverse primer	AGC GCA TAA ATC AAC CAA G
efm	E. faecium-specific cell wall adhesin	Forward primer	GAA AAG TTG TCA GTC GTG G	818
		Reverse primer	TGT TTG TGA CAA ACC TTC ATG
esp	Enterococcal surface protein	Forward primer	CAT CTT TGA TTC TTG GTT GTC G	695
		Reverse primer	GTT ATA GGT ACG TAT GTT GCA TCA
cylA	Hemolysin	Forward primer	GAG TTA GAT GAA TAT GGT CAT GGT	521
		Reverse primer	AGA AAC TAG CGA TGT AGG GTA ATA
cad1	Pheromone cAD1 precursor lipoprotein	Forward primer	TTC CAA AAC TAC GCA CAA CA	423
		Reverse primer	CTT TTT CAG CAG CAT TCA CTA ATT
ace	Collagen-binding cell wall protein	Forward primer	ATA GAA ACG GAT TTC GGA ACA G	298
		Reverse primer	TCA AAC TCG GCA AGT GAA ATA T
gelE	Gelatinase	Forward primer	TAT GAC AAT GCT TTT TGG GAT G	208
		Reverse primer	GCA CCC GAA ATA ATA TAA CCC
asa1	Aggregation substance	Forward primer	AAC AAG CTT GGT CTG TGT ATC	168
		Reverse primer	TCT TCC CCT TTC TTG TTA TGA AC